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KMID : 1041220030450020103
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Journal of Sericultural and Entomological Science
2003 Volume.45 No. 2 p.103 ~ p.107
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The Detection and Multiplicity of Entomopathogenic Nematodes Using Silkworms (Bombyx mori)
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Han Sang-Mi
Lee Kwang-Gil
Yeo Joo-Hong
Kweon Hae-Yong
Woo Soon-Ok
Lee Yong-Woo
Paik Ha-Ju
Han Myung-Se
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Abstract
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Entomopathogenic nematodes were isolated through the investigation in soils collected from cultivated and non-cultivated fields using silkworms (Bombyx mori) and Galleria mellonella trap. The detectable rate of entomopathogenic nematodes of silkworms trap was higher than the G. mellonella trap. This study indicates the detection of entomopathogenic nematodes from soils that silkworms are sensitive superior to the G. mellonella to entomopathogenic nematodes. The steinernema, rhabditidae, and diplogatroidae strains successfully cultured on the silkworms host as well as on artificial media. Reproductivity in the living silkworm larva and pupa was 1.5 to 3.5${\times}$ 10$\^$5/ nematodes per host However, G. mellonella could be multiplied less than 5${\times}$l0$\^$5/ nematodes. The dried pupa of the silkworm following mositurize was cultured 0.5 to 2${\times}$10$\^$5/) nematodes per host. The culture methods of the steinernema, rhabditidae, and diplogatroidae strains, using silkworm powder, extracted chicken intestine, and food waste fertilizer could be applicative, but rate of reproduction was low.
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KEYWORD
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silkworm, entomopathogenic nematodes, Galleria mellonella
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